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With the advent of sophisticated general programming environments like Mathematica, the task of developing new models of metabolism and visualizing their responses has become accessible to students of biochemistry and the life sciences in general. Modelling Metabolism with Mathematica presents the approaches, methods, tools, and algorithms for modelling the chemical-dynamics of metabolic pathways. The authors explain the concepts underpinning the deterministic theory of chemical and enzyme kinetics, present a graded series of computer models of metabolic pathways leading up to that of the human erythrocyte, and document a consistent set of rate equations and associated kinetic parameters.The experimental and theoretical study of metabolism in mammalian cells has a long and fruitful history, but our understanding of cellular metabolism at the molecular level is far from complete. This book enables its readers to formulate their own models of time-dependent metabolic systems and aids them in the quest for the many fundamental and clinically relevant discoveries that remain to be made. ... Read more

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This book deals with research on movement and locomotion and is designed to be a techniques book with the aim of providing methodological information about procedures, measurement and analysis. The first part of the book covers basic laboratory methodology for measurement of motor activity in a variety of animals as well as humans. The second section examines species-specific movements, including reflexes, reaching and grasping, swimming and locomotion. Both developmental and conceptual issues are included. The last part of the book provides an overview of statistical and experimental design issues to help the reader plan research projects and interpret findings. ... Read more

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Spanning biological, mathematical, computational, and engineering sciences, computational biofluiddynamics addresses a diverse family of problems involving fluid flow inside and around living organisms, organs, tissue, biological cells, and other biological materials. Computational Hydrodynamics of Capsules and Biological Cells provides a comprehensive, rigorous, and current introduction to the fundamental concepts, mathematical formulation, alternative approaches, and predictions of this evolving field.

In the first several chapters on boundary-element, boundary-integral, and immersed-boundary methods, the book covers the flow-induced deformation of idealized two-dimensional red blood cells in Stokes flow, capsules with spherical unstressed shapes based on direct and variational formulations, and cellular flow in domains with complex geometry. It also presents simulations of microscopic hemodynamics and hemorheology as well as results on the deformation of capsules and cells in dilute and dense suspensions. The book then describes a discrete membrane model where a surface network of viscoelastic links emulates the spectrin network of the cytoskeleton, before presenting a novel two-dimensional model of red and white blood cell motion. The final chapter discusses the numerical simulation of platelet motion near a wall representing injured tissue.

This volume provides a roadmap to the current state of the art in computational cellular mechanics and biofluiddynamics. It also indicates areas for further work on mathematical formulation and numerical implementation and identifies physiological problems that need to be addressed in future research. MATLAB^® code and other data are available at http://dehesa.freeshell.org/CC2 ... Read more

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In 1957 two young scientists, Matthew Meselson and Frank Stahl,produced a landmark experiment confirming that DNA replicates as predicted by thedouble helix structure Watson and Crick had recently proposed. It also gained immediaterenown as a "most beautiful" experiment whose beauty was tied to its simplicity. Yet theinvestigative path that led to the experiment was anything but simple, Frederic L. Holmesshows in this masterful account of Meselson and Stahl's quest.This book vividlyreconstructs the complex route that led to the Meselson-Stahl experiment and provides aninside view of day-to-day scientific research--its unpredictability, excitement,intellectual challenge, and serendipitous windfalls, as well as its frustrations, unexpecteddiversions away from original plans, and chronic uncertainty. Holmes uses research logs,experimental films, correspondence, and interviews with the participants to record thehistory of Meselson and Stahl's research, from their first thinking about the problemthrough the publication of their dramatic results. Holmes also reviews the scientificcommunity's reception of the experiment, the experiment's influence on laterinvestigations, and the reasons for its reputation as an exceptionally beautiful experiment. ... Read more

Customer Reviews (2)

A beautiful history of a beautiful experiment
This is a quite remarkable history.Holmes spent decades doing the research for this book.Even though I know both principals quite well and lived through their careers following the publication of the famous work, I did not know the full story of the work leading up to the "beautiful experiment".Holmes does a fantastic job capturing the thoughts and feelings of the principals and the supporting cast of Max Delbruck, Jim Watson, Cy Leventhal and others. Holmes thinks deeply about the philosophy of science and does a good job of explaining, with retroactive interviews, the thought processes of Meselson and Stahl as they circled around and finally hit on the right way to do the big experiment. The only small element I thought missing was a speculation about the possible reasons the Nobel committee has failed for more than 50 years to recognize the significance of the experiment.

How real science is done
This is an excellent book for anyone interested in knowing how real science is done. Real science is not the clean, simple, easy process described in the media. It is tedious, untidy, difficult and it takes patience, determination and passion. This book conveys an intimate and close view of how real scientific biological reaearch is conducted. ... Read more

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This book brings together current advances in embryonic, adult, neural, hematopoietic, mesenchymal and pancreatic stem cell functions. The chapters are authored by top-notch scientists in specialized areas of stem cell biology. Topics of the book focus on: Improved characterization of stem cells, Genomic alterations in stem cells during aging, Differential gene expression profiling during Quiescence, Self renewal and Differentiation, Stem Cell Transcriptomics and Proteomics, Regulatory feed back loops by stem cell transcription factors and post-transcriptional controls, Molecular Signaling Cross-talks in Stem cells, Transcriptional logics behind Nuclear Reprogramming, Clinical perspectives of Emerging Stem cell specific novel small RNAs (Piwi RNAs and MiRNAs), Epigenetic regulation and stem cell specification, and Stem cell niche and its functional significance in therapeutic interventions. The book will be an invaluable resource for researchers, scientists, and clinicians in the rapidly progressing field of stem cells and regenerative medicine.

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This book will be a resource for the experienced tissue engineer, a starting point for the student, and a guidebook for the next generation of tissue engineers. Contained in one volume is a comprehensive reference that combines the tools, experimental protocols, detailed descriptions, and "know-how" for the successful engineering of tissues and organs. The practical information contained in the numerous protocols covers every area of tissue engineering and will prove essential to scientists working in this field. Contributions by leaders in the latest areas of research will also be of interest to biotechnological and pharmaceutical researchers.


Key Features
* Provides comprehensive protocols covering every area of tissue engineering, including polymer synthesis, cell culture, encapsulation, bioreactors, therapeutics, and the creation of tissues and organs;
* Includes contributions by leaders in the latest areas of research, such as stem cells and fetal tissue engineering ... Read more

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RMIT Univ., Bundoora, Victoria, Australia. Presents state-of-the-art protocols of in situ hybridization. For cell or molecular biologists. Previous edition: c1994. ... Read more

Customer Reviews (1)

THE book for in situ methodologies
With the rapid and on-going development of In Situ hybridisation, a new edition of this book was needed. In Professor Darby the publishers found a very capable editor. He has gathered articles which, while conveying allthe protocols you would need to reproduce the techniques in your ownlaboratory, are surprisingly readable.

Even for the experiencedpractitioner, there are methods in here that will catch you by surprise.The wealth of illustrations add to the enjoyment, as you gain a very clearidea of what to expect. It is a shame, however, that most are in black andwhite. The supplement of colour photos (and the page in the professor's ownarticle) demonstrate the results most clearly.

As the summary says, ifyou are interested in hybridisation - whether of protein, chromosomes oreven whole embryos - this is an indispensable manual.

(caveat: probablynot a must-by for the general reader) ... Read more

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This book provides a foundation for understanding the fundamentals of biomedical informatics, which deals with the storage, retrieval, and use of biomedical data for biological problem solving and medical decision making. It covers the application of these principles to the three main biomedical domains of basic biology, clinical medicine, and public health. The author offers a coherent summary, focusing on the three core concept areas of biomedical data and knowledge representation, biomedical information access, biomedical decision making, and information and technology use in biomedical contexts.

* Develops principles and methods for representing biomedical data, using information in context and in decision making, and accessing information to assist the medical community in using data to its full potential

* Provides a series of principles for expressing biomedical data and ideas in a computable form to integrate biological, clinical, and public health applications

* Includes a discussion of user interfaces, interactive graphics, and knowledge resources and reference material on programming languages to provide medical informatics programmers with the technical tools to develop systems ... Read more

Customer Reviews (1)

Just what's needed
I have been teaching informatics for many years and found myself having to create lectures to provide deeper thinking skills about the contents of the field, without an organized text to support those topics. Every topic that informatics students should know is discussed in this text, examined from a deep perspective, and even accompanied by programming code that implements the idea. The programming language is Lisp. This is a language that is not used in most medical informatics production environments (like EHR vendors), but in fact represents the thought underlying most informatics applications. Read the book to be smarter. If you're a boundary-spanning informatician, you'll figure out how to communicate with folks who don't think the Lisp way. ... Read more

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Interest in the therapeutic value of embryonic, fetal and adult stem cell types is rapidly expanding throughout the scientific community.The first half of this century should see an explosion of therapeutic applications of stem cells which will grow from the cells and techniques described in this book.Stem Cell Culture provides a comprehensive resource for researchers in the fields of embryonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentiation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Chapters focus on the practical do's and don'ts of isolating and culturing these cell types, and use illustrative data or diagrams that allow the reader to confidently apply techniques and make this a standard reference.

*Compares and contrasts a broad range of species and stem cell types.
*Describes techniques in stem cell research, and goes beyond just protocols by presenting methods within perspective of recent literature reviews.
*Delineates critical steps and potential pitfalls for each method, provided by at-the-bench authors for expert information.
*Covers specific procedures in dealing with Human Embryonic Stem Cells, but also emphasizes other sources of potentially therapeutic stem cells such as amniotic fluid, adult muscle and adipose tissue, and umbilical cord. ... Read more

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Univ. of Newcastle upon Tyne, UK. Offers diagnostic laboratories, basic and medical researchers, and clinicians an authoritative collection of exacting diagnostic tools and research on the novel therapeutics now beginning to emerge. Features DNA and protein-based diagnosis. DNLM: Muscular Dystrophies--diagnosis. ... Read more

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A text intended for researchers and students in cell biology, genetics and molecular biology, developmental biology, biochemistry and biophysics. The complex problem of the relationship between nuclear structure and function demands a multidisciplinary, multifaceted approach. This laboratory guide is designed for researchers, from graduate students to professors, who need detailed protocols and general discussions on a broad range of techniques. The volume presents a selection of different methodological approaches for the analysis of nuclear structure and function - from cytological to molecular to genetic. These include visualization of nucleic acid sequences using hybridization probes, visualization of proteins using immunological probes, isolation of chromatin fractions, mapping "in vitro" protein-DNA interactions, reconstitution of functional templates and nuclear substructures, and genetic approaches to identifying and characterizing chromosomal components. ... Read more

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This cutting-edge book explores recent progress in the microelectronics arena. It examines the resultant miniaturization of component device features to nanometer size particles, and the ensuing growth in the development and use of microchip-based techniques in leading laboratories around the world. In the sixteen chapters of this volume, leading scientists present technically precise microchip-based assays for a variety of applications.

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This is essentially a work on marker substances useful for characterizing neurons and endocrine cells which incorporates the latest data on the molecular biology, biochemistry and cell biology of these marker molecules and explains how these molecules are employed clinically. It is also an attempt to bridge the gap between fundamental research and clinical practice. The clinical aspects on which the book concentrates principally concern abnormalities of the nervous and endocrine systems. Apart from the developmental relationships reviewed in the first chapter, neurons and endocrine cells share many common proteins which may in part constitute elements of the secretory machinery. Chapters 2 and 3 concentrate on clear synaptic vesicles and secretory granules, respectively, and focus attention in particular on either membrane constituents or proteins contained within granules. A class of molecule which profoundly affects both developmental events and pathological processes - the NCAM family - dealt with in Chapter 4, is representative of cell surface membrane constituents common to neural and endocrine cells.Chapter 5 discusses one of the first marker proteins used for identifying neurons and endocrine cells - the cytosolic enzyme neuron-specific enolase (NSE) - which was also one of the first to attain the status of a clinically useful diagnostic tool. Secretory proteins contained in granules, such as chromogranin A, and the marker substance synaptophysin are addressed in Chapters 6 and 7. The value of general cell markers, including intermediate filament proteins, and specific peptide hormone markers are also examined. ... Read more

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This up-to-date, authoritative laboratory manual can be used with the new edition of Biology, by Peter H. Raven and George B. Johnson, or with any general biology text. ... Read more

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Kings' College, London, UK. Laboratory manual providing methods for detection, isolation, identification, and characterization of mycoplasmas, with an emphasis on those of medical and veterinary significance. ... Read more

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International biobank collaborations allow for studies with large number of subjects where generalizability of findings across populations can be investigated, which means establishing quality criteria concerning the nature of the sample, conditions of sample storage, and the adequacy of available information is of vital importance. Methods in Biobanking brings together contributions from experts in the field in order to aid in the establishment of this much needed consistency. The volume discusses how to use existing collections of biological material to answer significant questions concerning the cause of disease without violating the personal integrity of participating sample donors, the ethical issues surrounding biobanks, guidelines for the use of coding systems and the use of biocomputing and registry linkages in research projects, as well as many other key subjects. As a volume in the highly successful Methods in Molecular Biology™ series, this collection provides the kind of detailed description and implementation advice that is crucial for getting optimal results.Authoritative and cutting-edge, Methods in Biobanking seeks to provide scientists with the tools necessary to take advantage of the tremendous current resources of the world’s biobanks and strengthen those resources for the future. ... Read more