Customer Reviews (2)

This is a must book for Histology
I really enjoyed the book. It goes very well with Histology courses.It gives you feeling of three dimensional pictures of real cells like photography. Since it is like a photography, it shows external views not the section throughs but you get hundered times better understanding of the structures. just look at scanning electron picture examples in your typical cell books. This book is full of them and not just plain pictures but with some explanations similar to Histology books.

an indispensable reference
This is a fantastic visual reference book for anybody interested in microscopic and/or macroscopic anatomy. Most of the major systems are covered, although the tissue samples are usually taken from small mammals.The range of magnification is great, and provides the reader with a clearconcept of the structure and function of a given organ.

I'm aprofessional medical illustrator with a library of over 300 books, and thisis one of only a few "desert island" books - ones that I wouldn'twant to be without. I believe anybody with an interest in the way we'remade will find it fascinating. ... Read more

Product Description
The modern electron microscope, as a result of recent revolutionary developments and many evolutionary ones, now yields a wealth of quantitative knowledge pertaining to structure, dynamics, and function barely matched by any other single scientific instrument. It is also poised to contribute much new spatially-resolved and time-resolved insights of central importance in the exploration of most aspects of condensed matter, ranging from the physical to the biological sciences. Whereas in all conventional EM methods, imaging, diffraction, and chemical analyses have been conducted in a static - time-integrated - manner, now it has become possible to unite the time domain with the spatial one, thereby creating four-dimensional (4D) electron microscopy. This advance is based on the fundamental concept of timed, coherent single-electron packets, or electron pulses, which are liberated with femtosecond durations. Structural phase transitions, mechanical deformations, and the embryonic stages of melting and crystallization are examples of phenomena that can now be imaged in unprecedented structural detail with high spatial resolution, and ten orders of magnitude as fast as hitherto. No monograph in existence attempts to cover the revolutionary dimensions that EM in its various modes of operation nowadays makes possible. The authors of this book chart these developments, and also compare the merits of coherent electron waves with those of synchrotron radiation. They judge it prudent to recall some important basic procedural and theoretical aspects of imaging and diffraction so that the reader may better comprehend the significance of the new vistas and applications now afoot. This book is not a vade mecum - numerous other texts are available for the practitioner for that purpose. It is instead an in-depth expose of the paradigm concepts and the developed techniques that can now be executed to gain new knowledge in the entire domain of biological and physical science, and in the four dimensions of space and time. ... Read more

Customer Reviews (1)

Important information, but there are "course prerequisites"
I discovered this book because it was referenced in a summary of the topic in Scientific American. I think the subject is important and fascinating. But after reading a few pages of the book, I decided regretfully that, as usual, before you can undertstand the latest scientific advances in any detail you have to master whole fields of science -- in this instance, optics and quantum physics. It's not the author's fault. He writes well, but there's no way to simplify the underlying prerequisites. (The annotated pictures in the Scientific American article did help to gain some notion of what's going on.)

... Read more

Product Description
This book covers the fundamentals of conventional transmission electron microscopy (CTEM) as applied to crystalline solids.The work is based on a lecture course given by Marc De Graef in the Department of Materials Science and Engineering at Carnegie Mellon University. ... Read more

Customer Reviews (2)

Wonderful Introduction to Transmission Electron Microscopy
For my taste, this book is the best introduction to Transmission Electron Microscopy that I have ever read.It is written at a higher level than Williams and Carter.This book is probably best suited to students with a B.S. in Physics.At the very least one should have benefited from a two semester undergraduate course on Modern Physics (including such things as solving the Schrodinger Equation for the Hydrogen atom) before tackling this book.I wish that a book like this had been out thirty years ago when I started learning TEM!This book does a very good job on two-beam theory and has truly wonderful dispersion surface diagrams.

Another excellent aspect of this book is its discussion of the Sturkey scattering matrix approach to modeling electron diffraction.Not only is the scattering matrix approach a good (and from my experience, often better) alternative to the numerical integration of the the Howie Whelan equations for simulating TEM images of extended defects, the author also shows how the scattering matrix approach can be used to derive Cowley-Moodie multislice theory.Indeed, the author's integration of these two different approaches to simulating TEM images, Howie-Whelan and multislice theory, within the context of the scattering matrix format, is extremely satisfying and illuminating!

The author also maintains a website of FORTRAN programs for download corresponding to the simulations described in the textbook.Furthermore, he is very forthcoming in providing guidance in finding the particular program that you are looking for.Even the source code is available for the perusal and use of the interested reader!

Excellent
I took Prof. de Graef's class at CMU in 2001, and we used pre-prints of this text.Even in that mostly-finished form, it was excellent.It's only improved now that I have the finished, published form.

Although I found the mathematical formalism that makes up the middle of this book excessive, it is important for people who will be modelling TEM experiments.The remainder of the book, on TEM basics, operation, and image/SADP interpretation, are all excellent.

This book is an excellent CTEM text. ... Read more

Customer Reviews (1)

Practical e- microscopy
Good book for practical applications of electron microscopy. Some stuff outdated (didn't use CCD cameras back then) but for the most part, still very applicable. ... Read more

Product Description
Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods.

Key Features
* Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized
* Describes methods that have been instrumental in exploring the three-dimensional structure of
* the nuclear pore complex
* the calcium release channel;
* the ribosome
* chaperonins ... Read more

Customer Reviews (1)

Crash Course in Single Particle Microscopy
This concise and easy to read book is essentialy a crash course in single particle reconstruction by electron microscopy.It isn't exactly a how-to manual, more an overview of how the whole process works.Thus, it is useful not only to someone who wants to learn how to do this, but also to those who might be interested in deciding if this technique would be useful to them.And actually, the whole topic is sufficiently cool that I would recommend the book even to people who know they will never use this technique, just because its fun to read about it.The book is clearly written and doesn't assume alot of prior knowledge.A basic undergrad education in math would be enough to handle the equations. ... Read more

Product Description
Histochemistry deals with the activities of chemical componentsin cells, and immunohistochemistry addresses the function of celltypes in tissue or organs, such as those leading to acceptance orrejection of grafts or organs. This book will be a methods volumefocusing on antigen retrieval, particularly methods used indisease-related antigens. Because the book is a methods volume and alab manual, it will have an audience of pathologists, biochemists, andlab technicians. ... Read more

Customer Reviews (1)

A brilliantly explained text
A brilliantly explained text containing extremely good applications, and above all, clear and well-referenced recipes. Most pitfalls in microscopy are the result of not following optimal processing conditions. After reading Dr. Hayat's present publication, there is no excuse for not following well-tested reliable protocols.

This Handbook is the most authoritative publication in the disciplines of microscopy, immunohistochemistry and antigen retrieval methods. It is expected to become a classic in teaching and research. This book should not be absent in your personal collection, as well at the library.

- Taken from a review by Prof. Dr. E. Marani, Afdeling Fysiologie, Leiden, The Netherlands. ... Read more

Product Description

• Offers a simple starting point to VPSEM, especially for new users, technicians and students containing clear, concise explanations
• Crucially, the principles and applications outlined in this book are completely generic: i.e. applicable to all types of VPSEM, irrespective of manufacturer.
• Information presented will enable reader to turn principles into practice
• Published in association with the Royal Microscopical Society (RMS) -www.rms.org.uk

Product Description

This two-volume Handbook is a comprehensive guide to sample preparation for the transmission electron microscope. Sample Preparation Handbook for Transmission Electron Microscopy: Volume 2: Techniques describes 14 different preparation techniques, including 22 detailed protocols for preparing thin slices for TEM analysis. Compatibility and pre-treatments are also discussed. Experimental conditions and guidelines, options and variations, advantages and constraints, technical hints from the authors’ years of experience, common artifacts, and theoretical issues are all considered. Particular attention is given to the type of material, conditioning, compatible analysis of a given preparation, and risks. This practical and authoritative reference companion deserves a place on the bench in every TEM lab.

Key Features of the Handbook:

• Combines all of the latest techniques for the preparation of mineral to biological samples
• Compares techniques in terms of their application areas, limitations, artifacts, and types of analysis (macroscopic, atomic, or molecular level)
• Describes physical characteristics, chemistry, structure/texture, and orientation properties of materials in relation to the most appropriate type of TEM analysis
• Links to a complementary interactive database website which is available to scientists worldwide*
• Written by authors with 100 years of combined experience in electron microscopy

*http://temsamprep.in2p3.fr/

Product Description
Introduction to Electron Microscopy for Biologists is ideal for the scientist who may be considering electron microscopy as a tool to extend molecular, biochemical, or light microscope observations to the next level of structural information, only available by electron microscopy.Each chapter briefly surveys the present state of structural information in a particular area, be it an individual but widely occurring molecule such as actin or collagen, together with the methods for visualization, either as an extracted and purified entity, or in situ within its biological context. Not only is this book an introduction to electron microscopy in general, but it is also useful for those within the field who wish to move to a different area of expertise, for instance an approach based on rapid freezing, rather than more conventional protocols.This should be a first choice reference for any biologist wanting to know 'what does it look like' across the full spectrum of cell and molecular biology of life science.

*Illustrates the advantages of electron microscopy and the protocols required for fine structural visualization of individual molecules upwards, ranging from mammalian tissues to experimental organisms.
*Comprehensive chapters cover both structural information on each area as well as the protocols themselves
*Includes methods covering specific labeling, as well as methods for quantification and three dimensional reconstruction from molecular to tissue levels. ... Read more

Customer Reviews (1)

Not an INTRODUCTION!!
This book claims to be an "Introduction" to electron microscopy, but it is not. Instead, it is a general description of VERY SPECIFIC methods, such as high pressure freezing, electron probe X-ray microanalysis, types of immunolabeling and specific techniques for each cellular organelle. IT DOES NOT CONTAIN ANY GENERAL METHOD OF PRINCIPLE, such as common fixatives, staining methods or embedding resins, nor does it say anything about the physics of the microscope.

If you are really looking for an INTRODUCTION (and a very general one, for that matter) to biological electron microscopy, I would recommend Bozzola's and Russell's Electron Microscopy. It contains general principles of both types of electron microscopes, basic techniques for standard methods, and chapters on more specific methods, like immunocytochemistry, autoradiography and freeze methods. For better and in-depth information about specimen preparation, Hayat's Principles and Techniques of Electron Microscopy is the best book available, although it does not contain any information on the physical principles of the electron micrsocope.

However, if your subject of interest are the physical principles of the electron microscope (and you are a biologist), although a bit old, Sjöstrand's book Electron Microscopy of Cells and Tissues is a very good reference, as well as Wischnitzer's Introduction to Electron Microscopy and Meek's Practical Electron Microscopy for Biologists. ... Read more

Product Description
While new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable.

The Handbook of Cryo-Preparation Methods for Electron Microscopy provides researchers with a complete reference that will show them how to equip their labs with the right materials and methods to take full advantage of the latest advances. This pioneering work brings together a group of internationally renowned researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations.

Contributions to this handbook –

Ö         Describe cryo-methods aimed at perfect preservation for fine structural analysis

Ö         Teach how to arrest physiological processes by cryo-fixation

Ö         Reveal the secrets for high-resolution snapshots of life by CEMOVIS

Ö         Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography

Ö         Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules

Ö         Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography

Ö         Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embedding

Ö         Highlight the Tokuyasu method and the new rehydration technique for highly efficient immunolabeling

Ö         Include summary tables to compare and obtain appropriate criteria

Includes a wealth of immediate reference material for daily use

With clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbookprovides researchers with the knowledge and techniques to adopt the latest cryo-methods to their current research. ... Read more

Product Description
This is an extensively illustrated laboratory manual of transmission electron microscopy techniques for the laboratory technician, graduate student, or researcher. Chapters begin with a general discussion, move on to the chemicals and equipment required for the method being described and conclude with a step-by-step presentation of the method and instructions for the preparation of solutions. Notes at the end of each chapter warn of possible pitfalls and outline "tricks of the trade". The methods and techniques outlined have been tested for over ten years in clinical and research laboratory situations, and are entirely reliable. Practical Electron Microscopy covers fixation, dehydration and embedding, semithin and thin sectioning, the electron microscope, and photography. For this new edition, the chapters on photography and the electron microscope have been completely rewritten and two new chapters have been added, one on immunoelectron microscopy using colloidal gold, and one dealing with such special techniques as retrieving specimens from paraffin and handling nasal brushings and blood samples. ... Read more

Customer Reviews (1)

A simple way to explain an complicated technique
I am a bigginer's in practicing the elelctron microscopy. This book provides a good introduction and important checklists during the practice of electron microscopy. Most important of all, it is not heavy. ... Read more

Product Description
This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists. It explains the mechanisms of image formation, contrast and its enhancement, and accounts for ultimate limits on the size of observable details (resolving power and resolution). Finally it provides an account of Fourier optical theory.Principles behind the photographic methods used in microscopy are also described and there is some discussion of image processing methods. Throughout, the text emphasizes the underlying similarity of all microscope systems and, recognizing that biologists may often be uncomfortable with mathematical approaches every effort has been made to present concepts verbally. ... Read more

Customer Reviews (1)

A superb job of scientific writing
I have looked at many microscope books in vain, before this one, to find clear explanations of the key topics in optical microscopy for my graduate students.None come close to competing with the Slayters.
Jerry Pine, Professor of Biophysics, Caltech ... Read more

Product Description
This completely revised new edition contains expanded coverage of existing topics and much new material. The author presents the subject of electron microscopy in a readable way, open both to those inexperienced in the technique, and also to practicing electron microscopists. He describes currently hot topics such as computer control of microscopes, energy-filtered imaging, cryomicroscopy and environmental microscopy, digital imaging, high resolution scanning, and transmission microscopy. The author has expanded the highly praised case studies of the first edition to include some interesting new examples. This indispensable guide to electron microscopy, written by an author with thirty years' practical experience, will be invaluable to new and experienced electron microscopists in any area of science and technology. ... Read more

Customer Reviews (1)

Not bad, a bit outdated
Read through this book for a TEM/Diffraction class. I'd call it a decent undergraduate-level introduction. Topics are introduced in very general terms with plenty of diagrams, plots, and images. Not extremely well organized, and very few equations. ... Read more

Customer Reviews (1)

A Classic Must Have
This book is a primer in SEM.It is a great shame it is out of print.Snatch up a used copy while you still can.If you have a used one of these that you don't have use for any longer.Sell it on marketplace. There are students every semester looking for this book. ... Read more