Product Description
Brand new book by two well-known experts in the field of microscopy; ; Replaces the best selling Introduction to the Optical Microscope by Savile Bradbury which was the first title in the Royal Microscopical Society's series of handbooks; ; Describes in s ... Read more

Product Description
Contrast in an image is essential to distinguish features from one another and from the background. This practical handbook describes the ways in which light interacts with the specimen in the microscope and the manner in which this is used to generate co ... Read more

Customer Reviews (1)

Very good (but concise) handbook. Recommend!
This book describes most (all?) of the known techniques of optical contrast enhancement in light microscopy. Enough information to fully understand the methods and at least some of their applications. I'd likemore details, but concede that it gives enough. Strongly recommended! ... Read more

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This book explains concepts of transmission electron microscopy (TEM) and x-ray diffractometry (XRD) that are important for the characterization of materials. The third edition has been updated to cover important technical developments, including the remarkable recent improvement in resolution of the TEM. This edition is not substantially longer than the second, but all chapters have been updated and revised for clarity. A new chapter on high resolution STEM methods has been added. The book explains the fundamentals of how waves and wave functions interact with atoms in solids, and the similarities and differences of using x-rays, electrons, or neutrons for diffraction measurements. Diffraction effects of crystalline order, defects, and disorder in materials are explained in detail. Both practical and theoretical issues are covered. The book can be used in an introductory-level or advanced-level course, since sections are identified by difficulty. Each chapter includes a set of problems to illustrate principles, and the extensive Appendix includes laboratory exercises.

Customer Reviews (4)

There are better books out there
I bought this book for a TEM/Diffraction class. A friend summed it up nicely: all the information you could want about TEM is in this book, but boy, do you have to dig for it.

Often rambling; hard to get the important concepts due to the large amount of verbose text. Problems in back of chapters often hard to solve unless you are making just the right assumptions. This is too bad, since Fultz's work is often interesting and his conversational style could be nice if it was reigned in a bit.

Get Willams and Carter for all practical purposes. The book from Goodhew, Humphreys, and Beanland is less mathematically rigorous than Fultz's but much more effective at getting concepts across. Egerton's book seems to be common, but I find it to be a bit too brief for me. There are a zillion TEM/Diffraction books out there, spend an afternoon in the library and choose one that suits your learning style.

Redudant and unclear
I cannot agree with the previous comments at all. Among all the books I've read in the area of materials characterization, this book is one of, if not, the most tedious one. Some concepts should have been explained in a much more concise and clear way. The description is very redundant and intimidating. The author fails to include more experimental contents but tells you where the sum of a geometric series comes from. Among many concepts and terms, the author smugly add much useless description which brings you nothing but headache. Other books, such as the famous TEM textbook by Williams and Carter, is obviously much much better than this one.

Excellent TEM book
This is now my go-to source for TEM information.Its very readable, yet has loads of information about the specific modes and analysis of TEM data.Its a good book to learn from and a pretty good reference.

wrote by TEM expert
This book is concise and comprehensive. Own it if you want to master TEM. ... Read more

Product Description
From the Preface: There has been a great upsurge in interest in light microscopy in recent years due to the advent of a number of significant advances in microscopy, one of the most important of which is confocal microscopy. Confocal microscopy has now become an important research tool, with a large number of new fluorescent dyes becoming available in the past few years for probing your pet structure or molecule within fixed or living cell or tissue samples. Many of the people interested in using confocal microscopy to further their research do not have a microscopy or even a cell biology background, and so not only do they find considerable difficulty in obtaining satisfactory results with a confocal, but they may be mislead by how data is being presented.This manual is intended to teach [its readers] the basic concepts of microscopy, fluorescence, digital imaging, and the principles of confocal microscopy so that [they] may take full advantage of the excellent confocal microscopes now available.This manual is an excellent reference source for information related to confocal microscopy for both beginners and more advanced users. The main emphasis in this manual in on the laser scanning confocal microscopes used in biology. However, a variety of related imaging technologies are briefly introduced as a comparison. ... Read more

Customer Reviews (2)

Very superficial
This book was recommended to me by the folks at the microscopy core in my university. I was hoping for an accessible technical primer on confocal microscopes with emphasis on the hardware and the underlying optical theory. Unfortunately, although there is an introduction to the hardware and a little bit of optics theory, these sections are very superficial and don't really add to your understanding of the technology (you'll be better off reading the the documentation on the manufacturer's websites, especially nikonU). The aim of this book seems to be to make sure you know what buttons to push when you are in front of the microscope, but not necessarily for you to understand what is that you are doing. The book is not bad. I would trade the section on software (that seems dated) for a good introduction to confocal optics and a more complete guide on how to make sure the hardware is optimally set up (key to get good images) which is also lacking.

An excellent introduction to practical confocal microscopy
This book provides a very "user-friendly" guide to beginners in the field of confocal microscopy. As introduction the author reviews in a compact and accesible manner basic issues of microscopy (including optics, the limits of microscopy, imaging modes and the parts of a microscope) and microscopy hardware always pointing out the advantages and drawbacks inherent to confocal microscopy. The main section of the book describes how to use a confocal microscope in detail, from collecting images to image presentation and publication, dealing along the way with topics such as interpretation of images, understanding fluorescence and fluorescent probes, specialized confocal microscopy techniques and live cell imaging. Appendices discuss further information that can be accessed through further reading or microscopy resources in the internet, as well as a succint evaluation of all commercial confocal microscopy brands currently available, highlighting their strengths and weaknesses.

The book has been written with a thorough care to produce an enjoyable and didactic text, containing many hints and "how to" procedures that have been personally very helpful to me when I started working with this technique, being completely novel to ANY class of microscopy.

The wealth of information available in its pages make it a good reference book on the subject. Indeed, we have adopted it in my laboratory as the standard for our confocal applications.

Highly recommended, in short. ... Read more

Product Description

The examination of structure at the microscopic scale, between micrometers and angstrom units, has changed dramatically in recent decades. Many new types of microscopy have emerged, notably the many scanning-probe designs, some of which also allow manipulation of atoms to form wanted structures, while others now permit direct observation of moving proteins in liquids. The traditional electron microscope is being revolutionized by the arrival of aberration correctors and monochromators, which bring the resolution below the Angstrom and electron-volt level. The "laboratory in a microscope" concept is rapidly evolving, as nanostructures are observed forming under controlled conditions at atomic resolution (the carbon nanotube being the most famous recent example). Electron holography and scanning transmission electron microscopy have become indispensable tools of the semiconductor industry. The oldest form of microscopy, optical microscopy, has been rejuvenated by the development of fluorescent, confocal, and two-photon variants. Analytical Scanning X-ray microscopes and Photoemission microscopes at synchrotons now routinely provide spatially resolved electronic structure maps. Tomographic imaging has vastly increased the information content of practically all forms of microscopy, as reflected in the award of a recent Nobel Prize. Molecular biology is benefiting enormously from progress in this technique. Most of these developments are responses to the urgent needs of researchers to characterize new useful nanostructures at the atomic level.

In Science of Microscopy, comprehensive reviews set these innovations in the context of microscopy today. Each contribution presents a form of microscopy or occasionally a microscopic technique, and provides information about the instruments involved and their areas of application. The contributions are written in such a way that the reader can understand how the various instruments function, their strengths and weaknesses, and whether they are suitable for a particular scientific investigation. Science of Microscopy will be an indispensable guide to both a wide range of scientists in university laboratories and to engineers and scientists in industrial R&D departments.

Key Features

• Full-length essays on each type of instrument or technique
• Applications to both materials science and the biomedical sciences
• Essay-length treatment by respected experts in each field
• Covers the latest developments as well as background information for the beginning microscopist

Product Description
Introduction to Optical Microscopy provides a rigorous and comprehensive overview of the fundamentals of optical microscopy. Starting from basic principles in Fourier optics, partial coherence, 3D imaging theory, and the physics of scattering and fluorescence, Introduction to Optical Microscopy explores a broad range of microscopy techniques. These include classical techniques such as phase contrast, confocal microscopy, etc., and progress to more modern techniques such as holographic microscopy, optical coherence tomography, two-photon microscopy, coherent anti-Stokes Raman scattering microscopy, etc.. The final chapters present a survey of new directions, including structured illumination and superresolution. Introduction to Optical Microscopy is designed to provide a solid theoretical foundation for graduate students or researchers who want to enter the field. While valuable as a reference, it can also serve as a textbook, featuring an updated Web site that provides problem sets and an instructor's solution manual. ... Read more

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This Handbook is a complete guide to preparing a wide variety of specimens for the scanning electron microscope and x-ray microanalyzer. Specimens range from inorganic, organic, biological, and geological samples to materials such as metals, polymers, and semiconductors which can exist as solids, liquids, and gases. While the Handbook complements the best-selling textbook, Scanning Electron Microscopy and X-Ray Microanalysis, Third Edition, by Goldstein, et al., it is entirely self-contained and describes what is needed up to the point the sample is put into the instrument. Photomicrographs of each specimen complement the many sample preparation “recipes”. Additional chapters describe the general features of specimen preparation in relation to the different needs of scanning electron microscopes and x-ray microanalyzers, and an appendix covers chemicals and equipment applicable to any of the recipes. This authoritative yet practical Handbook is an essential reference for anyone who uses these instruments, and assumes only an elementary knowledge of preparation techniques to guide the reader through the specific protocols.

Product Description
Atomic force microscopy (AFM) is part of a range of emerging microscopic methods for biologists which offer the magnification range of both the light and electron microscope, but allow imaging under the 'natural' conditions usually associated with the light microscope. To biologists, AFM offers the prospect of high resolution images of biological material, images of molecules and their interactions even under physiological conditions, and the study of molecular processes in living systems. This book provides a realistic appreciation of the advantages and limitations of the technique and the present and future potential for improving the understanding of biological systems. The second edition of this bestseller has been updated to describe the latest developments in this exciting field, including a brand new chapter on force spectroscopy. The dramatic developments of AFM over the past ten years from a simple imaging tool to the multi-faceted, nano-manipulating technique that it is today are conveyed in a lively and informative narrative, which provides essential reading for students and experienced researchers alike. ... Read more

Customer Reviews (2)

A powerful tool in biological sciences
Atomic force microscopy (AFM) is one technique in a family of new microscopies called scanning probe microscopies (SPMs) which has recently opened a wide range of novel, fascinating applications for biologists (biochemists, biophysicists, cell biologists, microbiologists,...). This book provides an excellent survey of those applications. It is nicely illustrated with numerous images from leading experts in the field. Clear descriptions of the apparatus and its basic principles are provided in a way accessible to students/scientists that do not have a strong physics background. This book will be useful to biologists, but also non-biologists dealing with biosystems, to evaluate the advantages and limitations of AFM in their specific field and to define appropriate procedures that will lead them to successful experiments.

Unravelling Biological Applications of SPM
"Atomic Force Microscopy for Biologists" is an excellent introduction for anybody wishing to enter this field. Nearly all aspects are covered. The book opens with an overview of the theory behind Atomic Force Microscopy and the different modes of use. This is presented in a concise manner without over-reliance on complex mathematical equations. Unlike many other volumes, practical advice on sample preparation techniques is provided for common systems such as proteins, cells and DNA. A comprehenive review of leading research in this area at the time of publication is sectioned by biomaterial type. Different approaches to imaging are tackled. The text is supported by numerous supporting images.

In summary I would strongly recommend this book to any biologist planning on carrying out research using SPM imaging technqiues and existing users in the field who wish to broaden their knowledge of SPM imaging and the research already carried out n common biological systems. ... Read more

Product Description

Scanning transmission electron microscopy has become a mainstream technique for imaging and analysis at atomic resolution and sensitivity, and the authors of this book are widely credited with bringing the field to its present popularity. Scanning Transmission Electron Microscopy(STEM): Imaging and Analysis will provide a comprehensive explanation of the theory and practice of STEM from introductory to advanced levels, covering the instrument, image formation and scattering theory, and definition and measurement of resolution for both imaging and analysis. The authors will present examples of the use of combined imaging and spectroscopy for solving materials problems in a variety of fields, including condensed matter physics, materials science, catalysis, biology, and nanoscience. Therefore this will be a comprehensive reference for those working in applied fields wishing to use the technique, for graduate students learning microscopy for the first time, and for specialists in other fields of microscopy.

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An up-to-date practical guide to the properties and characteristics of textile fibres, with clear advice on sampling, specimen preparation and examination procedures. ... Read more

Product Description

STEM is a discipline of importance to a growing number of microscopists. This book is essential reading for undergraduates, postgraduates and researchers requiring an up-to-date and comprehensive introduction to this rapidly growing, state of the art technique.

Customer Reviews (1)

Good, but not in-depth
This book gave me a quick and readable introduction to the STEM.Good work on the writers' part.

However, this is a short book, and doesn't go into much detail.It would be a good book for someone just starting out in STEM, but anyone with STEM experience might find only a few bits and pieces of the book enlightening. ... Read more

Product Description
The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections.

The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing. ... Read more

Product Description
Forensic Microscopy: A Laboratory Manual will provide the student with a practical overview and understanding of the various microscopes and microscopic techniques employed within the field of forensic science. Each laboratory experiment has been carefully designed to cover the variety of evidence disciplines within the forensic science field with carefully set out objectives, explanations of each topic and worksheets to help students compile and analyse their results.

The emphasis is placed on the practical aspects of the analysis to enrich student understanding through hands on experience. The experiments move from basic through to specialised and have been developed to cover a variety of evidence disciplines within forensic science field. The emphasis is placed on techniques currently used by trace examiners.

This unique, forensic focused, microscopy laboratory manual provides objectives for each topic covered with experiments designed to reinforce what has been learnt along with end of chapter questions, report requirements and numerous references for further reading. Impression evidence such as fingerprints, shoe tread patterns, tool marks and firearms will be analysed using simple stereomicroscopic techniques. Body fluids drug and trace evidence (e.g. paint glass hair fibre) will be covered by a variety of microscopes and specialized microscopic techniques. ... Read more

Product Description
The new edition of this comprehensive introductory text has been extensively revised and updated. The text is liberally illustrated and now incorporates questions and answers with each chapter. Already an established laboratory manual, this edition will be essential reading for both the materials scientist and the bio-scientist. ... Read more

Product Description
Originally published in 2005, this book covers the closely related techniques of electron microprobe analysis (EMPA) and scanning electron microscopy (SEM) specifically from a geological viewpoint. Topics discussed include: principles of electron-target interactions, electron beam instrumentation, X-ray spectrometry, general principles of SEM image formation, production of X-ray 'maps' showing elemental distributions, procedures for qualitative and quantitative X-ray analysis (both energy-dispersive and wavelength-dispersive), the use of both 'true' electron microprobes and SEMs fitted with X-ray spectrometers, and practical matters such as sample preparation and treatment of results. Throughout, there is an emphasis on geological aspects not mentioned in similar books aimed at a more general readership. The book avoids unnecessary technical detail in order to be easily accessible, and forms a comprehensive text on EMPA and SEM for geological postgraduate and postdoctoral researchers, as well as those working in industrial laboratories. ... Read more

Customer Reviews (1)

Excellent Book on the Basics
This book is great for someone who has a little background in physics and calculus. It gives a good breakdown of how the probe works and what kinds of analyses it works best for. This is not an in-depth book on quatitativeanalyses, but rather, an excellent place to start! ... Read more

Customer Reviews (2)

This is a must book for Histology
I really enjoyed the book. It goes very well with Histology courses.It gives you feeling of three dimensional pictures of real cells like photography. Since it is like a photography, it shows external views not the section throughs but you get hundered times better understanding of the structures. just look at scanning electron picture examples in your typical cell books. This book is full of them and not just plain pictures but with some explanations similar to Histology books.

an indispensable reference
This is a fantastic visual reference book for anybody interested in microscopic and/or macroscopic anatomy. Most of the major systems are covered, although the tissue samples are usually taken from small mammals.The range of magnification is great, and provides the reader with a clearconcept of the structure and function of a given organ.

I'm aprofessional medical illustrator with a library of over 300 books, and thisis one of only a few "desert island" books - ones that I wouldn'twant to be without. I believe anybody with an interest in the way we'remade will find it fascinating. ... Read more

Product Description
Although methods and techniques that will help solve various analytical problems do exist, they are often difficult to perform. Using polarized light microscopy as the method of choice, Color Atlas and Manual of Microscopy for Criminalists, Chemists, and Conservators offers swift, simple, yet irrefutable analytical tests and testing procedures that can be used to identify organic and inorganic particles. Seasoned forensic microscopists Nicholas Petraco and Thomas Kubic have lent their expertise as consultants to forensic scientists, analytical chemists, art historians, pathologists, customs agents, detectives, gemologists, numismatists, and art conservators. Now they share their extensive photomicrograph collection of minute specimens along with clear, concise, and simple methods to help solve your analytical problems. ... Read more

Customer Reviews (6)

Microscopy color atlas
This textbook is a must have for both practicing microscopists and students of microscopy.The text photographically illustrates proper techniques used by professional microscopists in a variety of microscopical applications.The references provided with each chapter provide a great avenue for further studies.I highly recommend this textbook to college professors who teach introductory or advanced techniques of microscopy.

Quick & comprehensive reference manual
This book is an extremely useful reference manual to have on hand for all microscopists.For the novice, it covers all the basics of microscopy that you will need to know.For the advanced students, it is a well-organized reference guide you will return to again and again.It's populated with lots of photographs and charts, and is an easy read.A definite must for any forensic criminalist's library!

Color Atlas and Manual of Microscopy for Criminalists, Chemists, and Conservators
I purchased this book for my husband, as he had previously seen excerpts and thought that the book would be good reference material. He certainly was not disappointed. The book gives clear and concise photographs and other documentation. It has proven helpful as a research resource, akin to "Google", just go to the Atlas and you're sure to find the information, or be pointed in the right direction.

Color Atlas of Microscopy
I highly recommend this book for both students enrolled in a college-level forensic microscopy course and for practicing forensic scientists as a desk reference.It is packed with hundreds of color photomicrographs that are referenced and described in the text.Diagrams assist in understanding light paths through specimens.Actual case studies from Petraco and Kubic integrate the theoretical concepts with practical applications.

Good printing - substandard content
The book is apparently put together from a couple of PowerPoint presentations. The printed graphics are partly very poor and commonly unsystematic, rarely explaining the point they attempt to make. References to standard text books and further studies are inadequate. The frequent and unnecessary use of abbreviations makes some chapters difficult to read. Some technical mistakes and misleadingly unprecise text passages have been spotted.
This book is too confusing for the novice of the field who just wants only an introduction and much too light-weight and superficial for the advanced reader. ... Read more

Product Description
Histochemistry deals with the activities of chemical componentsin cells, and immunohistochemistry addresses the function of celltypes in tissue or organs, such as those leading to acceptance orrejection of grafts or organs. This book will be a methods volumefocusing on antigen retrieval, particularly methods used indisease-related antigens. Because the book is a methods volume and alab manual, it will have an audience of pathologists, biochemists, andlab technicians. ... Read more

Customer Reviews (1)

A brilliantly explained text
A brilliantly explained text containing extremely good applications, and above all, clear and well-referenced recipes. Most pitfalls in microscopy are the result of not following optimal processing conditions. After reading Dr. Hayat's present publication, there is no excuse for not following well-tested reliable protocols.

This Handbook is the most authoritative publication in the disciplines of microscopy, immunohistochemistry and antigen retrieval methods. It is expected to become a classic in teaching and research. This book should not be absent in your personal collection, as well at the library.

- Taken from a review by Prof. Dr. E. Marani, Afdeling Fysiologie, Leiden, The Netherlands. ... Read more