Product Description
This book covers the fundamentals of conventional transmission electron microscopy (CTEM) as applied to crystalline solids.The work is based on a lecture course given by Marc De Graef in the Department of Materials Science and Engineering at Carnegie Mellon University. ... Read more

Customer Reviews (2)

Wonderful Introduction to Transmission Electron Microscopy
For my taste, this book is the best introduction to Transmission Electron Microscopy that I have ever read.It is written at a higher level than Williams and Carter.This book is probably best suited to students with a B.S. in Physics.At the very least one should have benefited from a two semester undergraduate course on Modern Physics (including such things as solving the Schrodinger Equation for the Hydrogen atom) before tackling this book.I wish that a book like this had been out thirty years ago when I started learning TEM!This book does a very good job on two-beam theory and has truly wonderful dispersion surface diagrams.

Another excellent aspect of this book is its discussion of the Sturkey scattering matrix approach to modeling electron diffraction.Not only is the scattering matrix approach a good (and from my experience, often better) alternative to the numerical integration of the the Howie Whelan equations for simulating TEM images of extended defects, the author also shows how the scattering matrix approach can be used to derive Cowley-Moodie multislice theory.Indeed, the author's integration of these two different approaches to simulating TEM images, Howie-Whelan and multislice theory, within the context of the scattering matrix format, is extremely satisfying and illuminating!

The author also maintains a website of FORTRAN programs for download corresponding to the simulations described in the textbook.Furthermore, he is very forthcoming in providing guidance in finding the particular program that you are looking for.Even the source code is available for the perusal and use of the interested reader!

Excellent
I took Prof. de Graef's class at CMU in 2001, and we used pre-prints of this text.Even in that mostly-finished form, it was excellent.It's only improved now that I have the finished, published form.

Although I found the mathematical formalism that makes up the middle of this book excessive, it is important for people who will be modelling TEM experiments.The remainder of the book, on TEM basics, operation, and image/SADP interpretation, are all excellent.

This book is an excellent CTEM text. ... Read more

Product Description
The modern electron microscope, as a result of recent revolutionary developments and many evolutionary ones, now yields a wealth of quantitative knowledge pertaining to structure, dynamics, and function barely matched by any other single scientific instrument. It is also poised to contribute much new spatially-resolved and time-resolved insights of central importance in the exploration of most aspects of condensed matter, ranging from the physical to the biological sciences. Whereas in all conventional EM methods, imaging, diffraction, and chemical analyses have been conducted in a static - time-integrated - manner, now it has become possible to unite the time domain with the spatial one, thereby creating four-dimensional (4D) electron microscopy. This advance is based on the fundamental concept of timed, coherent single-electron packets, or electron pulses, which are liberated with femtosecond durations. Structural phase transitions, mechanical deformations, and the embryonic stages of melting and crystallization are examples of phenomena that can now be imaged in unprecedented structural detail with high spatial resolution, and ten orders of magnitude as fast as hitherto. No monograph in existence attempts to cover the revolutionary dimensions that EM in its various modes of operation nowadays makes possible. The authors of this book chart these developments, and also compare the merits of coherent electron waves with those of synchrotron radiation. They judge it prudent to recall some important basic procedural and theoretical aspects of imaging and diffraction so that the reader may better comprehend the significance of the new vistas and applications now afoot. This book is not a vade mecum - numerous other texts are available for the practitioner for that purpose. It is instead an in-depth expose of the paradigm concepts and the developed techniques that can now be executed to gain new knowledge in the entire domain of biological and physical science, and in the four dimensions of space and time. ... Read more

Customer Reviews (1)

Important information, but there are "course prerequisites"
I discovered this book because it was referenced in a summary of the topic in Scientific American. I think the subject is important and fascinating. But after reading a few pages of the book, I decided regretfully that, as usual, before you can undertstand the latest scientific advances in any detail you have to master whole fields of science -- in this instance, optics and quantum physics. It's not the author's fault. He writes well, but there's no way to simplify the underlying prerequisites. (The annotated pictures in the Scientific American article did help to gain some notion of what's going on.)

... Read more

Product Description
The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided.

The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques. ... Read more

Product Description

This book presents recent advances in the field of nanoscale characterization of ferroelectric materials using scanning probe microscopy (SPM). It addresses various imaging mechanisms of ferroelectric domains in SPM, quantitative analysis of the piezoresponse signals as well as basic physics of ferroelectrics at the nanoscale level, such as nanoscale switching, scaling effects, and transport behavior. This state-of-the-art review of theory and experiments on nanoscale polarization phenomena will be a useful reference for advanced readers as well for newcomers and graduate students interested in the SPM techniques. The non-specialists will obtain valuable information about different approaches to electrical characterization by SPM, while researchers in the ferroelectric field will be provided with details of SPM-based measurements of ferroelectrics.

Product Description
In Unbiased Steriology, the authors present a practical guide to making unbiased 3D measurements using microscopy. Stereology is the statistical inference of geometric parameters from spatially sampled information; it is considered unbiased whent he test probes are positioned in an appropriately random manner. Now that unbiased stereology is required for nerve-cell counts in neurotoxicology by the FDA and by many journals before the acceptance of an article or study, this book is essential reading. It includes only those stereological techniques that have been tried and tested by actual application. Although these techniques are essentially mathematical--unbiased stereology is a quantitative rather than qualitative approach to microscopy-the authors do not immerse the reader in complex theories. Instead, they provide simple heuristic explanations and references to the original proof, illustrating the theory with analogies draw from everyday experience. Exercises-provided at the end of each chapter, complete with detailed worked answers-have been developed by the authors or donated by other expert stereologists to give practical experience using the techniques. Specific topis covered include: random sampling and random geometry; the estimation of feature volume, surface, length, number and mean particle size; as well as statistics for stereologists. ... Read more

Customer Reviews (1)

Extremely useful for all interested in true quantification
This is an excellent book for all those interested in true quantification of tissue elements. Here, methods are outlined, amply illustrated and put forward in the form of exercises making it a pleasure even for the novicereader to get an understanding of what stereology is and what it is for.Now, the day seems near when researchers report their results onquantitative tissue features based on stereological estimation procedures.Highly recommended for serious morphologists. ... Read more

Product Description
Optical Microscopy: Emerging Methods and Applications covers recent technical advances and new approaches to monitoring and altering cell physiology, examining membrane cytoarchitecture, observing multiple cellular activities and intact organ physiology, plus confocal imaging of live cell function, lifetime imaging, and automated clinical imaging cytometry. The book provides the reader with a synopsis of the most recent technical developments in optical microscopy as applied to scientific research. Each chapter introduces new methods by describing how these overcome limitations inherent in previous techniques. Software, hardware, and other equipment concerns are covered. Additionally, the book reviews current applications in order to stimulate future developments in optical microscopy, encouraging novel uses and new technical advances

Key Features
Includes comprehensive descriptions of
* Caged compounds, fluorescence ratio imaging, and CCD video cameras
* Simultaneous multiple detection and real-time fluorescence microscopy
* Simultaneous DIC and quantitative LLF video imaging
* Total internal reflectance, time-resolved, and automated fluorescence microscopy
* Laser-scanning confocal microscopy
* Imaging for calcium measurements, membranes, glycoproteins, living cells, and cancer cells ... Read more

Product Description
Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are. ... Read more

Customer Reviews (1)

Crash Course in Single Particle Microscopy
This concise and easy to read book is essentialy a crash course in single particle reconstruction by electron microscopy.It isn't exactly a how-to manual, more an overview of how the whole process works.Thus, it is useful not only to someone who wants to learn how to do this, but also to those who might be interested in deciding if this technique would be useful to them.And actually, the whole topic is sufficiently cool that I would recommend the book even to people who know they will never use this technique, just because its fun to read about it.The book is clearly written and doesn't assume alot of prior knowledge.A basic undergrad education in math would be enough to handle the equations. ... Read more

Product Description
Visualization of nucleic acids has become indispensable to studying cells, tissues, and organisms. Certain techniques even permit quantification of DNA and/or RNA distribution in tissues, but few current analytical books cover the numerous methods for DNA and RNA visualization. This book provides insight into several classic techniques, histological as well as histochemical, that can be used to appreciate the nucleic acid status of the cell as well as to provide an overview of RNA and DNA distribution in cells and tissues.Genome Visualization by Classic Methods in Light Microscopy begins with an introduction to DNA and RNA, followed by general visualization principles.The subsequent chapters describe: how to prepare tissues for staining; the principles, chemical formulas, and procedures for nuclear dye, fluorescent dye, and histochemical methods; directions to observe the products of the stained reactions; and more.Each protocol is presented as easy-to-follow directions and the author includes cautionary notes and points to consider.The final section provides color photographs of various tissues in which the staining method, fixative, and observations are noted.A theoretical and practical book, Genome Visualization by Classic Methods in Light Microscopy allows you to understand which technique is most useful for your particular problem.Laboratory protocols are provided for you to follow, chemical structures and principles are provided for you to understand the technique, and the book is organized so you can find the necessary information when needed.This is the essential guide to understanding and executing visualization techniques for nucleic acids. ... Read more

Product Description

This two-volume Handbook is a comprehensive guide to sample preparation for the transmission electron microscope. Sample Preparation Handbook for Transmission Electron Microscopy: Volume 2: Techniques describes 14 different preparation techniques, including 22 detailed protocols for preparing thin slices for TEM analysis. Compatibility and pre-treatments are also discussed. Experimental conditions and guidelines, options and variations, advantages and constraints, technical hints from the authors’ years of experience, common artifacts, and theoretical issues are all considered. Particular attention is given to the type of material, conditioning, compatible analysis of a given preparation, and risks. This practical and authoritative reference companion deserves a place on the bench in every TEM lab.

Key Features of the Handbook:

• Combines all of the latest techniques for the preparation of mineral to biological samples
• Compares techniques in terms of their application areas, limitations, artifacts, and types of analysis (macroscopic, atomic, or molecular level)
• Describes physical characteristics, chemistry, structure/texture, and orientation properties of materials in relation to the most appropriate type of TEM analysis
• Links to a complementary interactive database website which is available to scientists worldwide*
• Written by authors with 100 years of combined experience in electron microscopy

*http://temsamprep.in2p3.fr/

Product Description
Transmission Electron Microscopy presents the theory of image and contrast formation, and the analytical modes in transmission electron microscopy. The principles of particle and wave optics of electrons are described. Electron-specimen interactions are discussed for evaluating the theory of scattering and phase contrast. Also discussed are the kinematical and dynamical theories of electron diffraction and their applications for crystal-structure analysis and imaging of lattices and their defects. X-ray micronanalysis and electron energy-loss spectroscopy are treated as analytical methods. Specimen damage and contamination by electron irradiation limits the resolution for biological and some inorganic specimens. This fifth edition includes discussion of recent progress, especially in the area of aberration corrector, and energy filtering; moreover the new topics of the fourth edition have been updated again. ... Read more

Customer Reviews (1)

microstructure
inspectuon of iron and iron alloy by optical micro scopy and scaning electron microscopy? ... Read more

Product Description
This completely revised new edition contains expanded coverage of existing topics and much new material. The author presents the subject of electron microscopy in a readable way, open both to those inexperienced in the technique, and also to practicing electron microscopists. He describes currently hot topics such as computer control of microscopes, energy-filtered imaging, cryomicroscopy and environmental microscopy, digital imaging, high resolution scanning, and transmission microscopy. The author has expanded the highly praised case studies of the first edition to include some interesting new examples. This indispensable guide to electron microscopy, written by an author with thirty years' practical experience, will be invaluable to new and experienced electron microscopists in any area of science and technology. ... Read more

Customer Reviews (1)

Not bad, a bit outdated
Read through this book for a TEM/Diffraction class. I'd call it a decent undergraduate-level introduction. Topics are introduced in very general terms with plenty of diagrams, plots, and images. Not extremely well organized, and very few equations. ... Read more

Product Description
A practical introduction to basic theory and contemporary applications across a wide range of research disciplines

Over the past two decades, scanning probe microscopies and spectroscopies have gained acceptance as indispensable characterization tools for an array of disciplines. This book provides novices and experienced researchers with a highly accessible treatment of basic theory, alongside detailed examples of current applications of both scanning tunneling and force microscopies and spectroscopies.

Like its popular predecessor, Scanning Probe Microscopy and Spectroscopy, Second Edition features contributions from distinguished scientists working in a wide range of specialties at university, commercial, and government research labs around the world. Chapters have been edited for clarity, conciseness, and uniformity of presentation to provide professionals with a concise working reference to scanning probe microscopic and spectroscopic principles, techniques, and practices.

This Second Edition has been substantially revised and expanded to reflect important advances and new applications. In addition to numerous examples, the Second Edition features expanded coverage of electrostatic and magnetic force microscopies, near-field optical microscopies, and new applications of buried interfaces in nanomechanics, electrochemistry, and biology.

Scanning Probe Microscopy and Spectroscopy, Second Edition is an indispensable working resource for surface scientists, microscopists, and spectroscopists in materials science, chemistry, engineering, biochemistry, physics, and the life sciences. It is also an unparalleled reference text for advanced undergraduates and graduate students in those fields. ... Read more

Customer Reviews (1)

A good reference on STM
This book mainly focus on STM.However, it doesn't cover much on AFM.The use of AFM has become increasingly popular in recent years in research investigation in various areas, including cell biology, DNA research, material science, nanotechnology, and so on.The editor may consider include detailed discussion on AFM in next edition (if any). ... Read more

Product Description

• Offers a simple starting point to VPSEM, especially for new users, technicians and students containing clear, concise explanations
• Crucially, the principles and applications outlined in this book are completely generic: i.e. applicable to all types of VPSEM, irrespective of manufacturer.
• Information presented will enable reader to turn principles into practice
• Published in association with the Royal Microscopical Society (RMS) -www.rms.org.uk

Product Description
Atom-probe field ion microscopy is currently the only technique capable of imaging solid surfaces with atomic resolution, and at the same time of chemically analyzing surface atoms selected by the observer from the field ion image. Field ion microscopy has been successfully used to study most metals and many alloys, and recently good field ion images of some semiconductors and even ceramic materials such as high temperature superconductors have been obtained. Although other microscopies are capable of achieving the same resolution, there are some experiments unique to field ion microscopy--for example the study of the behavior of single atoms and clusters on a solid surface. The very elegant development of the field ion microscope with the atom-probe has provided a powerful and useful technique for highly sensitive chemical analysis. This book presents the basic principles of atom-probe field ion microscopy and illustrates the various capabilities of the technique in the study of solid surfaces and interfaces at atomic resolution. ... Read more

Product Description
While new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable.

The Handbook of Cryo-Preparation Methods for Electron Microscopy provides researchers with a complete reference that will show them how to equip their labs with the right materials and methods to take full advantage of the latest advances. This pioneering work brings together a group of internationally renowned researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations.

Contributions to this handbook –

Ö         Describe cryo-methods aimed at perfect preservation for fine structural analysis

Ö         Teach how to arrest physiological processes by cryo-fixation

Ö         Reveal the secrets for high-resolution snapshots of life by CEMOVIS

Ö         Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography

Ö         Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules

Ö         Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography

Ö         Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embedding

Ö         Highlight the Tokuyasu method and the new rehydration technique for highly efficient immunolabeling

Ö         Include summary tables to compare and obtain appropriate criteria

Includes a wealth of immediate reference material for daily use

With clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbookprovides researchers with the knowledge and techniques to adopt the latest cryo-methods to their current research. ... Read more

Product Description

Microscopy, which has served as a fundamental scientific technique for centuries, remains an invaluable tool in chemistry, biology, healthcare, and forensics. Increasingly, it is being integrated into modern chemical instrumentation and is of value as a powerful analytical tool across many scientific disciplines.

Designed to serve as a primary resource for undergraduate or graduate students, An Introduction to Microscopy helps students master the foundational principles of microscopy. Intentionally concise, this text does not attempt to cover all aspects of all types of microscopy such as polarizing light and fluorescence. Instead, the authors’ intent is to provide students with the basic knowledge necessary to explore and understand these more advanced techniques. The authors draw from their own extensive backgrounds in forensic identification to explain the methods and ways in which microscopy shapes every investigation.

All nine chapters include questions and most include simple exercises related to the material covered. Numerous figures and photographs supplement the text and explain the procedures and principles introduced. A glossary is included as well as a convenient list of abbreviations, and references to more in-depth readings.

Offers a Fundamental Approach for Students in all Fields

The material assumes basic mathematics skill through algebra and a basic knowledge of fundamental chemistry and physics (essential for understanding optics). Although the authors used the high-quality microscopes found in their laboratories to produce the images found in the book, the information and methods can be applied to any type of microscope to which students have access.

Understanding the fundamentals of microscopy provides students with a relevant and marketable skill that can be readily applied in many fields, even if the students have not had significant academic training in the subject. Furthermore, by understanding various aspects of microscopy, students will begin to understand the science behind other related areas, such as spectroscopy, optics, and any number of applications involving analytical instrumentation.

Product Description

The volumes VIII, IX and X examine the physical and technical foundation for recent progress in applied scanning probe techniques. The first volume came out in January 2004, the second to fourth volumes in early 2006 and the fifth to seventh volumes in late 2006. The field is progressing so fast that there is a need for a set of volumes every 12 to 18 months to capture latest developments. These volumes constitute a timely comprehensive overview of SPM applications. After introducing scanning probe microscopy, including sensor technology and tip characterization, chapters on use in various industrial applications are presented. Industrial applications span topographic and dynamical surface studies of thin-film semiconductors, polymers, paper, ceramics, and magnetic and biological materials. The chapters have been written by leading researchers and application scientists from all over the world and from various industries to provide a broader perspective.