Product Description
Most information about the nature of living cells is discovered through light microscope observation. The image seen through a microscope is not a magnified point-by-point representation of the object, but is an abstract representation of the object that must be interpreted. This interpretation is influenced not only by the actual structure of the object, but the physical characteristics of light and the microscope components, the preparation of the sample, and the microscopist's background. In order to interpret the image correctly and fully, the microscopist must understand the interaction of light with the sample.

The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells which influence interpretation.

* Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope

* Clearly develops all ideas from historical and logical foundations

* Laboratory exercises included to assist the reader with practical applications

* Microscope discussions include: bright field microscope, dark field microscope, oblique illumination,phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope
... Read more

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The only updated resource on acoustic microscopy covers its use in a range of applications spanning the fields of physics, materials science, electrical engineering, medicine, and research and industry.

Acoustic Microscopy offers detailed coverage of:

• acoustic field structure
• output signal formation in transmission raster acoustic microscopes
• non-linear acoustic effects
• visco-elastic properties and microstructure of model systems and composites
• polymer composite materials
• microstructure and physical-mechanical properties of biological tissues
• and more

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Filling a gap in the literature, this book features in-depth discussions on amplitude modulation AFM, providing an overview of the theory, instrumental considerations and applications of the technique in both academia and industry. As such, it includes examples from material science, soft condensed matter, molecular biology, and biophysics, among others. The text is written in such a way as to enable readers from different backgrounds and levels of expertise to find the information suitable for their needs. ... Read more

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This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists. It explains the mechanisms of image formation, contrast and its enhancement, and accounts for ultimate limits on the size of observable details (resolving power and resolution). Finally it provides an account of Fourier optical theory.Principles behind the photographic methods used in microscopy are also described and there is some discussion of image processing methods. Throughout, the text emphasizes the underlying similarity of all microscope systems and, recognizing that biologists may often be uncomfortable with mathematical approaches every effort has been made to present concepts verbally. ... Read more

Customer Reviews (1)

A superb job of scientific writing
I have looked at many microscope books in vain, before this one, to find clear explanations of the key topics in optical microscopy for my graduate students.None come close to competing with the Slayters.
Jerry Pine, Professor of Biophysics, Caltech ... Read more

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Scanning tunneling microscopy has achieved remarkable progress and become the key technology for surface science. This book predicts the future development for all of scanning probe microscopy (SPM). Such forecasts may help to determine the course ultimately taken and may accelerate research and development on nanotechnology and nanoscience, as well as all in SPM-related fields in the future.

Product Description
In his book Karl Mahall describes the damage which can occur in certain fibrous raw materials and during production and storage of textiles; for this purpose he has carefully chosen typical practical examples which he encountered in connection with textile auxiliaries during the 40 years which he has been working for the Henkel KGaA. In particular it is demonstrated how microscopic test methods can provide decisive hints at the cause of hidden faults in textiles. Each damage detection case is illustrated by photos. This book will be useful as a manual and reference book for all people in industry, trade and education who have to deal with damage detection and quality assurance of textiles as well as all other textile experts who want to get an insight into this special field. ... Read more

Product Description
Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manuals Cells: A Laboratory Manual and Live Cell Imaging provide a solid course in the basics of using the microscope in a biology laboratory. Basic Methods in Microscopy provides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events. This manual is an important tool for any biology researcher employing imaging as a research method. Related Titles from the Publisher Live Cell Imaging: A Laboratory Manual Cells: A Laboratory Manual Molecular Cloning: A Laboratory Manual Imaging in Neuroscience and Development: A Laboratory Manual Lab Math Lab Ref, Volume 2: A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench Lab Dynamics: Management Skills for Scientists At the Bench At the Helm ... Read more

Customer Reviews (1)

A book with useful protocols
This is a book covering the principles of all kinds of microscopes with structure figures. There many good color photos and graphs. The protocols of tissue preparations are very useful, butmaybe duplicate in other books. If you just want to know how to use the microscopes instead of understanding them, it should be a good book for you. It is more like a lab manual collection.

The problem of this book is that it distracts a little bit from microscopes to cell culture and immunohistochemistry. ... Read more

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This fully updated edition surveys both old and new techniques, including chromosome microscopy, immunohistochemistry, fluorescence microscopy, image building, video microscopy, calcium and pH imaging, microinjection techniques, nanovid microscopy, and histomorphometry. ... Read more

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Near-field optics studies the behaviour of light fieldsin the vicinity of matter, where light is structured in propagatingand evanescent fields. Near-field optical microscopy is thestraightforward application of near-field optics.

This textbook provides an overview for undergraduates and anyone whohas an interest in peculiar optical phenomena, and serves as atechnical manual for engineers and researchers. It consists of 12chapters dealing with the history of near-field optics, non-radiatingoptics, optical noise, inverse problems, theory, instrumentation andapplications; there is an appendix including the basic elements ofFourier optics and Maxwell equations. ... Read more

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The investigation and manipulation of matter on the atomic scale have been revolutionized by scanning tunneling microscopy and related scanning probe techniques. This book is the first to provide a clear and comprehensive introduction to this subject. Beginning with the theoretical background of scanning tunneling microscopy, the design and instrumentation of practical STM and associated systems are described in detail, including topographic imaging, local tunneling barrier height measurements, tunneling spectroscopy, and local potentiometry. A treatment of the experimental techniques used in scanning force microscopy and other scanning probe techniques rounds out this section.The second part discusses representative applications of these techniques in fields such as condensed matter physics, chemistry, materials science, biology, and nanotechnology, so this book will be extremely valuable to upper-division students and researchers in these areas. ... Read more

Customer Reviews (2)

My opinion about"Scannig Probe Microscopy and Spectroscopy".
This book is one of the principal lecturesthat every research need to understand "Scanning probe apparatus". Its content refer all the different areas of actual interest and the different type of measurements that actually could be make with this type of system. This book is more than a simple introduction in this wonderful area of physics. For all this reasons I thing that is one of the most important books that every research have to read.

Poor print quality
The price of this paperback of SPM book is very good compared to that of the hardcover. However, the print quality is poor. In some of the images you barely see the featutres they talk about in the book. I am a SPM user so this is really bothering me. The SPM imagaes in this book are not very informative due to the poor print quality. Maybe the hardcover one has better print quality. ... Read more

Product Description
This text describes the most important techniques for the study of opaque minerals. It covers the basic techniques of sample preparation and examination using polished sections and the polarized light microscope. The qualitative properties used for mineral identification, including textures, are examined, and numerous examples of the major mineral associations are provided. This edition has been updated to reflect new technical and theoretical developments, including new data on the most important ore types. Tables of data for the identification of the most common ore minerals are provided in the appendix. ... Read more

Customer Reviews (1)

Practical e- microscopy
Good book for practical applications of electron microscopy. Some stuff outdated (didn't use CCD cameras back then) but for the most part, still very applicable. ... Read more

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By using nanotechnological methods, we can now poke around protein molecules, genes, membranes, cells and more. Observation of such entities through optical and electron microscopes tempt us to touch and manipulate them. It is now possible to do so, and scientists around the world have started pulling, pushing and cutting small structures at the base of life processes to understand the effect of our hand work.

The book describes the physical properties of such life supporting structures from the molecular level with a special emphasis on their designs based on the mechanical strength and flexibility, membrane and other biological nanostructures.


- Describes the basic mechanical features of proteins, DNA, cell membrane and other biological nanostructures
- Explains the basic concepts and mathematics of elementary mechanics needed to understand and perform experimental work ... Read more

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This book presents a unified view of the rapidly growing field of scanning tunneling microscopy and its many derivatives. After examining novel scanning-probe techniques and the instrumentation and methods, the book provides detailed accounts of STM applications. It examines limitations of the present-day investigations and provides insight into further trends.
"I strongly recommend that Professor Bai's book be a part of any library that serves surface scientists, biochemists, biophysicists, material scientists, and students of any science or engineering field...There is no doubt that this is one of the better (most thoughtful) texts."
Journal of the American Chemical Society (Review of 1/e) ... Read more

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Since 1995, the noncontact atomic force microscope (NC-AFM) has achieved remarkable progress. Based on nanomechanical methods, the NC-AFM detects the weak attractive force between the tip of a cantilever and a sample surface. This method has the following characteristics: it has true atomic resolution; it can measure atomic force interactions, i.e. it can be used in so-called atomic force spectroscopy (AFS); it can also be used to study insulators; and it can measure mechanical responses such as elastic deformation. This is the first book that deals with all of the emerging NC-AFM issues.

FROM THE REVIEWS:

MATERIALS TODAY"This book gives a comprehensive overview of the state-of-the-art of this dynamic force microscopy technique in 20 chapters, each written by experts in the field. It covers the theoretical basis, as well as applications to semiconducting surfaces, ionic crystals, metal oxides, and organic molecular systems including thin films, polymers, and nucleic acids . . . There are unsolved questions about the mechanisms responsible for atomic resolution but, as this well-written book displays, there has been tremendous progress in basic understanding of the technique and fascinating new applications continue to arise . . . With an increased understanding of NC-AFM, as demonstrated in this book, we are certain to see further progress in the near future." ... Read more

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Introduction to Electron Microscopy for Biologists is ideal for the scientist who may be considering electron microscopy as a tool to extend molecular, biochemical, or light microscope observations to the next level of structural information, only available by electron microscopy.Each chapter briefly surveys the present state of structural information in a particular area, be it an individual but widely occurring molecule such as actin or collagen, together with the methods for visualization, either as an extracted and purified entity, or in situ within its biological context. Not only is this book an introduction to electron microscopy in general, but it is also useful for those within the field who wish to move to a different area of expertise, for instance an approach based on rapid freezing, rather than more conventional protocols.This should be a first choice reference for any biologist wanting to know 'what does it look like' across the full spectrum of cell and molecular biology of life science.

*Illustrates the advantages of electron microscopy and the protocols required for fine structural visualization of individual molecules upwards, ranging from mammalian tissues to experimental organisms.
*Comprehensive chapters cover both structural information on each area as well as the protocols themselves
*Includes methods covering specific labeling, as well as methods for quantification and three dimensional reconstruction from molecular to tissue levels. ... Read more

Customer Reviews (1)

Not an INTRODUCTION!!
This book claims to be an "Introduction" to electron microscopy, but it is not. Instead, it is a general description of VERY SPECIFIC methods, such as high pressure freezing, electron probe X-ray microanalysis, types of immunolabeling and specific techniques for each cellular organelle. IT DOES NOT CONTAIN ANY GENERAL METHOD OF PRINCIPLE, such as common fixatives, staining methods or embedding resins, nor does it say anything about the physics of the microscope.

If you are really looking for an INTRODUCTION (and a very general one, for that matter) to biological electron microscopy, I would recommend Bozzola's and Russell's Electron Microscopy. It contains general principles of both types of electron microscopes, basic techniques for standard methods, and chapters on more specific methods, like immunocytochemistry, autoradiography and freeze methods. For better and in-depth information about specimen preparation, Hayat's Principles and Techniques of Electron Microscopy is the best book available, although it does not contain any information on the physical principles of the electron micrsocope.

However, if your subject of interest are the physical principles of the electron microscope (and you are a biologist), although a bit old, Sjöstrand's book Electron Microscopy of Cells and Tissues is a very good reference, as well as Wischnitzer's Introduction to Electron Microscopy and Meek's Practical Electron Microscopy for Biologists. ... Read more

Product Description
Scanning Probe Microscopy - Analytical Methods provides a comprehensive overview of the analytical methods on the nanometer scale based on scanning probe microscopy and spectroscopy. Numerous examples of applications of the chemical contrast mechanism down to the atomic scale in surface physics and chemistry are discussed with extensive references to original work in the recent literature. ... Read more

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Univ. of Milan, Italy. Provides hands-on protocols for using atomic force microscopy (AFM) in biomedical research. Each tested protocol includes detailed instructions, background material, tips for troubleshooting, and notes on now to distinguish artifacts from useful data. For researchers. DNLM: Microscopy Atomic Force--methods. ... Read more