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Saccharomyces cerevisiae is an important research tool and industrial reagent. This organism is the basis for much of our understanding of the molecular and cellular biology of eukaryotes. The second edition of this authoritative reference updates certain chapters and includes new chapters on aging and the cell wall. With a wealth of new research data collected since the first edition, The Metabolism and Molecular Physiology of Saccharomyces cervisiae remains a leading reference for bioscientists across many disciplines. ... Read more

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In this second volume of a three-volume set (updating the seminal 1981 monograph The Molecular Biology of the Yeast Saccharomyces), the emphasis is on gene expression. A continuing theme of these monographs has been the extent to which basic biological processes have been conserved in the eukaryotic ... Read more

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At a fundamental research level, the yeasts offer valuableopportunities for modelling regulatory and metabolic processes inmulticellular eukaryotic organisms: this volume deals with themultifunctional chromosome regulatory proteins, topoisomerase andnuclear transport. A combination of biochemical and genetic approachesapplied to the yeast translation system is also currently yielding awealth of data, while the mating pheromone signal transduction pathwayin yeasts provides a valuable analogue of the signal transductioncomponents used by multicellular organisms, including receptors, Gproteins, protein kinases and transcription factors.
With a well-established history of fermantation studies, yeasts remainthe first-choice vehicle for production of heterologous eukaryoticproteins. Interest is diversifying, as an increasing number ofnon-Saccharomyces species are now being utilised for theproduction of specific heterologous proteins.
Molecular biologists, microbiologists and biochemical geneticists willfind this volume an authoritative and valuable update on a vibrantarea of research.
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In all eukaryotic cells, each fundamental process cycle progression and its control, protein secretion and targeting, transcription and its regulation, mRNA processing, and DNA replication accomplished by essentially identical cellular machinery composed of essentially identicalprotein components. This conservation of function has catapulted the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe from parochial backwaters to the forefront of experimental molecular biology: What is true for a yeast is true for an elephant, and in experiments you can get the answer a lot faster from a yeast. This burgeoning appreciationof yeasts as model systems for the study of fundamental cellular processeshas highlighted the need for an update of the seminal 1981 monograph The Molecular Biology of the Yeast Saccharomyces. This need is now met by the publication of a three-volume series to serve as the authoritative sequel.The first volume focuses on the genome organization of the yeast Saccharomyces as well as protein translation and its regulation and energymetabolism. Subsequent volumes emphasize such topics as the cell cycle, secretion, and transcription. Together, these volumes provide a comprehensive survey of the molecular and cellular biology of Saccharomyces and Schizosaccharomyces, serving not only as a current summary of every significant area of investigation, but also as a thoroughreference source. These volumes are required reading for everyone in the field and anyone curious about the state of the art of molecular and cellular biology. ... Read more

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Nowhere in science is the relationship betweenfundamental and applied research as evident as inyeast research. Saccharomyces cerevisiae is the yeastresponsible for alcoholic fermentation, and has beenused for centuries as mankind´s oldest domesticatedorganism in wine making, baking, brewing anddistilling. This species became the model organism“par excellence”, was the first eukaryotic genome tobe sequenced, and stands at the forefront ofmolecular biology and functional analysis in geneticsand genomics. The market value of products derivedfrom fermentations with S. cerevisiae is expected toincrease much above the general market growth in thefuture. This book presents an assessment of moleculartechniques for accurate genotyping of S. cerevisiaestrains and a three-years biogeographical andpopulation genetics survey of S. cerevisiae strainsfrom winemaking environments, for the conservation ofbiodiversity and sustainable development of geneticresources. ... Read more

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Chapters: Saccharomyces Boulardii, Saccharomyces Cerevisiae, Candida, Ashbya Gossypii, Brettanomyces, Pichia Pastoris, Saccharomyces Pastorianus, Saccharomyces Kluyveri, Brettanomyces Bruxellensis, Saccharomycetaceae, Pichia Stipitis, Zygosaccharomyces, Saccharomycetales, Nematospora Coryli, Kluyveromyces Marxianus, Ascobotryozyma, Starmerella, Pichia Guilliermondii, Geotrichum Candidum Var. Citri-Aurantii, Saccharomyces Bayanus, Yamadazyma, Torulaspora Delbrueckii, Geotrichum Candidum, Saccharomycodaceae, Debaryomyces, Dipodascaceae, Endomycetaceae, Lipomycetaceae, Starmera, Kodamaea, Saccharomycopsidaceae, Hyphopichia, Eremotheciaceae, Nakazawaea, Phaffomyces, Cephaloascaceae, Zygosaccharomyces Bailii, Ascoideaceae, Kluyveromyces Lactis, Kawasakia, Ascocephalophora, Phialoascus, Sporopachydermia, Trichomonascaceae, Yarrowia, Pichiaceae, Galactomyces, Dipodascopsis, Geotrichum Klebahnii, Endomyces, Lipomyces, Zygozyma, Metschnikowiaceae, Dipodascus, Eremothecium, Coccidiascus, Pichia Membranifaciens, Zygosaccharomyces Florentinus, Saccharomyces Florentinus, Galactomyces Candidum, Pichia Subpelliculosa, Hansenula Subpelliculosa. Source: Wikipedia. Pages: 174. Not illustrated. Free updates online. Purchase includes a free trial membership in the publisher's book club where you can select from more than a million books without charge. Excerpt: Saccharomyces boulardii (brand name Florastor, among others) is a tropical strain of yeast first isolated from lychee and mangosteen fruit in 1923 by French scientist Henri Boulard. It is related to, but distinct from, Saccharomyces cerevisiae in several taxonomic, metabolic, and genetic properties. S. boulardii has been shown to maintain and restore the natural flora in the large and small intestine; it is classified as a probiotic. Boulard first isolated the yeast after he observed natives of Southeast Asia chewing on the skin of lychee and mangosteen in an attempt to control the symp...More: http://booksllc.net/?id=8459450 ... Read more

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Chapters: Antibodies, Antivenom, Anti-Transglutaminase Antibodies, Anti-Gliadin Antibodies, Anti-Saccharomyces Cerevisiae Antibodies, Anti-Ganglioside Antibodies, Intravenous Immunoglobulin, Anti-Citrullinated Protein Antibody, Autoantibody, Immunoglobulin E, Anti-Topoisomerase Antibodies, Anti-Apolipoprotein Antibodies, Immunoglobulin A, Anti-Nuclear Antibody, Neutralizing Antibody, Immunoglobulin G, Biolegend, Immunoglobulin M, Immunoglobulin D, Anti-Glutamate Receptor Antibodies, Heavy Chain Antibody, Anti-Cardiolipin Antibodies, Anti-Glycoprotein-210 Antibodies, Anti-Mitochondrial Antibody, Anti-Cholesterol, Anti-Actin Antibodies, Anti-Streptolysin O, Idiotopes, Anti-P62 Antibodies, Anti-Centromere Antibodies, Monospecific Antibody, Cr6261, Immunoglobulin Y, Anti-Smooth Muscle Antibody, Neutralisation. Source: Wikipedia. Pages: 133. Not illustrated. Free updates online. Purchase includes a free trial membership in the publisher's book club where you can select from more than a million books without charge. Excerpt: Antivenom (or antivenin or antivenene) is a biological product used in the treatment of venomous bites or stings. Antivenom is created by injecting a small amount of the targeted venom into an animal such as a horse, sheep, goat, or rabbit; the subject animal will undergo an immune response to the venom, producing antibodies against the venom's active molecule which can then be harvested from the animal's blood and used to treat envenomation. Internationally, antivenoms must conform to the standards of Pharmacopoeia and the World Health Organization (WHO). The name antivenin comes from the French word venin, meaning venom, and historically antivenin was predominant around the world. In 1981, the World Health Organization decided that the preferred terminology in the English language would be "venom" and "antivenom" rather than "venin/antivenin" or "venen/antivenene". The principle of antivenom is based on that...More: http://booksllc.net/?id=391320 ... Read more

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This digital document is a journal article from DNA Repair, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

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Replication forks can stall spontaneously at specific sites in the genome, and upon encountering DNA lesions resulting from chemical or radiation damage. In Saccharomyces cerevisiae proteins implicated in processing of stalled replication forks include those encoded by the SGS1, TOP3, MUS81, MMS4, SLX1, SLX4, SLX5/HEX3, and SLX8 genes. We tested the roles of these genes in suppressing gross chromosomal rearrangements (GCRs), which include translocations, large interstitial deletions, and loss of a chromosome arm with de novo telomere addition. We found that mus81, mms4, slx1, slx4, slx5, and slx8 mutants all have elevated levels of spontaneous GCRs, and that SLX5 and SLX8 are particularly critical suppressors of GCRs during normal cell cycle progression. In addition to increased GCRs, deletion of SLX5 or SLX8 resulted in increased relocalization of the DNA damage checkpoint protein Ddc2 and activation of the checkpoint kinase Rad53, indicating the accumulation of spontaneous DNA damage. Surprisingly, mutants in slx5 or slx8 were not sensitive to transient replication fork stalling induced by hydroxyurea, nor were they sensitive to replication dependent double-strand breaks induced by camptothecin. This suggested that Slx8 and Slx8 played limited roles in stabilizing, restarting, or resolving transiently stalled replication forks, but were critical for preventing the accumulation of DNA damage during normal cell cycle progression. ... Read more

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This digital document is a journal article from Bioresource Technology, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

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Biodegradation and decolorization of monosodium glutamate wastewater were carried out by using an acidophilus yeast strain of Saccharomyces cerevisiae and Coriolus versicolor. For the yeast treatment, the highest COD removal and reducing sugar removal efficiency were 76.6% and 80.2%, respectively. The color removal was only 2%. For C. versicolor treatment, the highest COD removal, color removal and reducing sugar removal efficiencies were 78.7%, 56.5% and 90.9%, respectively. The synergic treatment process, in which the yeast and C. versicolor were successively applied,exhibited great advantage over the individual process. ... Read more

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This is the third volume in a set designed to provide a comprehensive survey of the molecular and cellular biology of saccharomyces and schizosaccharomyces. ... Read more

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This digital document is a journal article from Bioresource Technology, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

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Fermentation of sugar by Saccharomyces cerevisiae, for production of ethanol in an immobilized cell reactor (ICR) was successfully carried out to improve the performance of the fermentation process. The fermentation set-up was comprised of a column packed with beads of immobilized cells. The immobilization of S. cerevisiae was simply performed by the enriched cells cultured media harvested at exponential growth phase. The fixed cell loaded ICR was carried out at initial stage of operation and the cell was entrapped by calcium alginate. The production of ethanol was steady after 24 h of operation. The concentration of ethanol was affected by the media flow rates and residence time distribution from 2 to 7 h. In addition, batch fermentation was carried out with 50 g/l glucose concentration. Subsequently, the ethanol productions and the reactor productivities of batch fermentation and immobilized cells were compared. In batch fermentation, sugar consumption and ethanol production obtained were 99.6% and 12.5% v/v after 27 h while in the ICR, 88.2% and 16.7% v/v were obtained with 6 h retention time. Nearly 5% ethanol production was achieved with high glucose concentration (150 g/l) at 6 h retention time. A yield of 38% was obtained with 150 g/l glucose. The yield was improved approximately 27% on ICR and a 24 h fermentation time was reduced to 7 h. The cell growth rate was based on the Monod rate equation. The kinetic constants (K"s and @m"m) of batch fermentation were 2.3 g/l and 0.35 g/lh, respectively. The maximum yield of biomass on substrate (Y"X"/"S) and the maximum yield of product on substrate (Y"P"/"S) in batch fermentations were 50.8% and 31.2% respectively. Productivity of the ICR were 1.3, 2.3, and 2.8 g/lh for 25, 35, 50 g/l of glucose concentration, respectively. The productivity of ethanol in batch fermentation with 50 g/l glucose was calculated as 0.29 g/lh. Maximum production of ethanol in ICR when compared to batch reactor has shown to increase approximately 10-fold. The performance of the two reactors was compared and a respective rate model was proposed. The present research has shown that high sugar concentration (150 g/l) in the ICR column was successfully converted to ethanol. The achieved results in ICR with high substrate concentration are promising for scale up operation. The proposed model can be used to design a lager scale ICR column for production of high ethanol concentration. ... Read more

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This digital document is a journal article from DNA Repair, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

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SMC6 (RHC18) in Saccharomyces cerevisiae, which is a homologue of the Schizosaccharomyces pombe rad18^+ gene and essential for cell viability, encodes a structural maintenance of chromosomes (SMC) family protein. In contrast to the rest of the SMC family of proteins, Smc1-Smc4, which are the components of cohesin or condensin, little is known about Smc6. In this study, we generated temperature sensitive (ts) smc6 mutants of budding yeast and characterized their properties. One ts-mutant, smc6-56, ceased growth soon after up-shift to a non-permissive temperature, arrested in the late S and G2/M phase, and gradually lost viability. smc6-56 cells at a permissive temperature showed a higher sensitivity than wild-type cells to various DNA damaging agents including methyl methanesulfonate (MMS). The rad52 smc6-56 double mutant showed a sensitivity to MMS similar to that of the rad52 single mutant, indicating that Smc6 is involved in a pathway that requires Rad52 to function. Moreover, no induction of interchromosomal recombination and sister chromatid recombination was observed in smc6-56 cells, which occurred in wild-type cells upon exposure to MMS. ... Read more

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Chapters: Yeast, Saccharomyces Boulardii, Candida Albicans, Killer Yeasts, Saccharomyces Cerevisiae, Malassezia, Fungal Prions, Schizosaccharomyces Pombe, Hansenula Polymorpha, Yeast Expression Platform, Brettanomyces, Cryptococcus Neoformans, Pichia Pastoris, Saccharomyces Pastorianus, Candida Dubliniensis, Candida Glabrata, Nutritional Yeast, Fleischmann's Yeast, Saccharomyces Cerevisiae Virus L-A, Brettanomyces Bruxellensis, Candida Krusei, Malt Granules, Saccharomycetaceae, National Collection of Yeast Cultures, Pichia Stipitis, Zygosaccharomyces, Candida Lusitaniae, Saccharomycotina, Saccharomycetales, Kluyveromyces Marxianus, Candida Parapsilosis, Red Star Yeast, Rhodotorula, Pichia Guilliermondii, Arming Yeast, Saccharomyces Bayanus, Torulaspora Delbrueckii, Debaryomyces, Zygosaccharomyces Bailii, Saccharomycetes, Candida Tropicalis, Metschnikowiaceae, Candida Stellata, Candida Viswanathii, Schizosaccharomycetales, Candida Oleophila, Schizosaccharomycetes, Candida Milleri, Schizosaccharomycetaceae, Pachysolen Tannophilus, Scoby. Source: Wikipedia. Pages: 202. Not illustrated. Free updates online. Purchase includes a free trial membership in the publisher's book club where you can select from more than a million books without charge. Excerpt: Ascomycota Basidiomycota Yeasts are eukaryotic micro-organisms classified in the kingdom Fungi, with the 1,500 species currently described estimated to be only 1% of all yeast species. Most reproduce asexually by budding, although a few do so by binary fission. Yeasts are unicellular, although some species with yeast forms may become multicellular through the formation of a string of connected budding cells known as pseudohyphae, or false hyphae, as seen in most molds. Yeast size can vary greatly depending on the species, typically measuring 34 µm in diameter, although some yeasts can reach over 40 µm. The yeast species Saccharomyces cerevisiae has been used in baking and fermentin...More: http://booksllc.net/?id=34385 ... Read more

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Chapters: Yeast, Sodium Bicarbonate, Leavening Agent, Saccharomyces Cerevisiae, Sourdough, Baking Powder, Baker's Yeast, Proofing, Potassium Carbonate, Potassium Bitartrate, Fleischmann's Yeast, Bread Improver, Glucono Delta-Lactone, Pre-Ferment, Biga, Barm, Red Star Yeast, Desem, Levain, Clabber Girl, Chef. Source: Wikipedia. Pages: 105. Not illustrated. Free updates online. Purchase includes a free trial membership in the publisher's book club where you can select from more than a million books without charge. Excerpt: Ascomycota Basidiomycota Yeasts are eukaryotic micro-organisms classified in the kingdom Fungi, with the 1,500 species currently described estimated to be only 1% of all yeast species. Most reproduce asexually by budding, although a few do so by binary fission. Yeasts are unicellular, although some species with yeast forms may become multicellular through the formation of a string of connected budding cells known as pseudohyphae, or false hyphae, as seen in most molds. Yeast size can vary greatly depending on the species, typically measuring 34 µm in diameter, although some yeasts can reach over 40 µm. The yeast species Saccharomyces cerevisiae has been used in baking and fermenting alcoholic beverages for thousands of years. It is also extremely important as a model organism in modern cell biology research, and is one of the most thoroughly researched eukaryotic microorganisms. Researchers have used it to gather information about the biology of the eukaryotic cell and ultimately human biology. Other species of yeast, such as Candida albicans, are opportunistic pathogens and can cause infections in humans. Yeasts have recently been used to generate electricity in microbial fuel cells, and produce ethanol for the biofuel industry. Yeasts do not form a single taxonomic or phylogenetic grouping. The term "yeast" is often taken as a synonym for Saccharomyces cerevisiae, but the phylogenetic diversity of yeasts is...More: http://booksllc.net/?id=34385 ... Read more